Protocols in Production Sequencing
Libraries
Large Clone Isolation Protocol
DNA Quantification Protocol
Library Creation
- General 3 kb Protocol
Vector: pUC18; restriction site: CCCGGG; sequence - Mid-size 8-10 kb Protocol
Vector: pMCL200; restriction site: GATATC; sequence (Gene 162, 157-158, 1995.) - Fosmid 40 kb Protocol
Vector: pCC1Fos; restriction site: CACGTG; sequence
Library Plating Protocol
Q-Pix Colony Picker Protocol
Fosmid DNA Inoculation Protocol
Fosmid DNA Isolation Protocol
Sequencing Preparation
Rolling Circle Amplification Protocol using TempliPhi
Sequencing Chemistry
- ET Terminator Automated Protocol
- Big Dye 3.1 Sequencing of Fosmid DNA Protocol
- Big Dye 3.1 Cycle Sequencing Protocol
Sequencers
ABI3730xl Users Protocol
MegaBACE 4500 Users Protocol
