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… of secondary metabolism. Welcome back. This will be episode 12 of Natural Prodcast. And it’s our conversation with … Europe. So if there are any kind of dated references to the news, that’s why. But it was a really fun conversation. And …

… getting them out to you all. But for now, here is episode 10 of Natural Prodcast, the primer on genome mining. Hey, … in Yu et al, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2004, 1253–1262, 70:3 ALISON TAKEMURA: And so did you succeed in … Microbiomes” paper is here . Read the associated JGI news release here . Software we discussed, used for genome …

… so there are– I hesitate to make a guess but maybe 50 to 100 different strains and they are in individual tubules.  … in the field, these glacial bacteria grow slow and it’s not 100% clear that we know what their biological activities … call this the needle in the Haystack. Because if we have 10,000 features which one do we look at because we are a …

… the Molecular Foundry, the project needs to be scoped for 12 months or less. We can help you scope that part of the proposal. Also, once awarded, users have 12 months before they need to activate their project. … end of an active proposal in order to continue access in 12-month increments.  At the JGI, a typical proposal is …

… there a way to do that? DAN UDWARY: Well, so there’s good news and bad news on that. So one of the things that we’re … happening. And so we should have somewhere around 10, 11, 12 million BGCs in the database on the release, and that’s …

… right? BEN: Yeah, yeah. Well, we are at [10 to the] minus 12 [Molar concentration]- is sufficient to kill cells. So … So I wanted to ask you also about – because you were in the news recently – TSRI has acquired the Pfizer strain …

… convince those people of that fact, however, a long time, 10 years! ALISON TAKEMURA: Wow. It’s all happened so fast. I … of the molecules in ecology. And that took another 10 years at least. But I had several students. And I worked … course using modern phylogenetic tools measuring genes, the 16S gene, and comparing it with everything that’s known. So …

… people what that number is. BETSY PARKINSON: So micromolar, 10 to the negative 6th. Nanomolar, 10 to the negative 9th. Picomolar, 10 to the negative 12th. DAN: Yeah. So very-- BETSY PARKINSON: Very, very-- … them back to us and we'll do a PCR analysis to sequence the 16S and things like that to figure out what they actually …

… first started out the natural product community was almost 100% bacterial. Only people working in mycotoxins worked … do you check if they’re there? Like we always talk about 16S for bacteria, but we don’t talk about it so easily with … mostly coming out of China and India, very cheap for $100-$200. But you have so many contigs. This is a problem …

… as well as a unique opportunity to complete a California K-12 science or math teaching credential as an undergraduate …